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The aim of this project
is to develop a genetic transformation system for cowpeas.
The longer-term goal is the introduction of insect
resistance genes into cowpea.
A wide range of cowpea genotypes,
including large seeded- and forage types, were multiplied in order to
have sufficient seeds for tissue culture purposes. Compared to the old
seed batches, newly grown seeds showed high germination rates (up to
96%) and no microbial contamination when germinated in-vitro. The old
seed batches germinated very poorly, if at all, and usually led to
tissue cultures that were 100% contaminated.
Over 20 different cowpea genotypes
are being analysed for their their tissue culture response in-vitro.
Regeneration of multiple shoots is set as a prerequisite for genetic
transformation experiments. Based on early results, several cultivars
look promising for further work and will be used for transformation
experiments mediated by Agrobacterium tumefaciens.
Gene vectors are being constructed
with a suitable selectable marker gene and access to insecticidal genes
is at an advanced stage of negotiation.
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