|
PI:
|
Idah Sithole-Niang
|
Contact details
Department of Biochemistry
P.O. MP 167, Mt. Pleasant,
Harare, Zimbabwe
Phone: +263 (-4) -303211 ,
Ext 1663
Fax: +263 (-4) -333407
/333678
Email direct from site:
isn@mweb.co.zw
|
|
Grantee:
|
University of Zimbabwe
|
|
Amount:
|
US $87,150
|
|
Duration:
|
Three Years |
|
Collaborator:
Dr. Richard F Allison, Plant
Biology, Michigan State University
|
|
|
-
Generated a workable and novel
genetic transformation system of cowpea based on:
-
enzymatic expression of the
marker gene GUS by ELISA detection,
-
PCR of the transgene (coat
protein gene), and
-
a positive hybridization event
on Southern blots. However no sequencing has been done to verify
the insertion sites, and no virus challenge has been conducted to
confirm resistance as yet.
-
Four transgenic cowpeas
(Zimbabwean cultivar 475/89) identified by the above criteria and
awaiting evaluation for virus resistance
-
Capacity building:
-
infrastructure development:
computer and gel documentation system, flourimeter, incubator
shaker and the growth chambers acquired
-
human resource development:
further training for the student and collaboration with Michigan
State University
-
Successful negotiation of IPR
waiver on patented technology, the first ever negotiated in
sub-Saharan Africa, and no money spent on the process
-
Scientific publications envisaged
-
|
|
