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Resistance to African cassava mosaic virus conferred by a mutant of the
putative NTP-binding domain of the rep gene (AC1) in Nicotiana benthamiana
A. Sangaré
We
constructed a mutation in DNA-A of African Cassava Mosaic Virus (ACMV)
to alter the putative NTP-binding domain site in the
replication-associated protein gene (AC1). When transgenic Nicotiana
benthamiana plants expressing the mutated gene were infected with
ACMV, the plants exhibited tolerance to infection, consisting in a delay
in symptom appearance and/or the presence of mild symptoms. In addition,
the resistant plants accumulated less viral DNA than non-transgenic
palnts. As judged by northern blot analysis and symptom development of
segregating progeny from different lines, a high level of expression of
the mutant is essential for the development of resistance. Issues
related to the use of different versions of AC1 for the control of ACMV
are discussed.
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