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Biotechnology, Breeding and Seed Systems for African Crops

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Biotechnology Research Abstracts from the Biotechnology, Breeding and Seed Systems conference


 Striga free maize: Introducing Tripsacum-based resistance via an integrated approach

 S.J. Hearne, A.L. Gurney J.P. Brizard, C. Brugidou, F. Kanampiu, P.O. Mbogo, M.C. Press, J.D. Scholes & D. Hoisington

Tetraploid Tripsacum dactyloides (L.) exhibits resistance and tolerance to Striga under field and laboratory conditions.  The development of Striga is impaired soon after attachment.  This perturbation of Striga development has been observed in crosses between maize and tetraploid Tripsacum indicating that the trait may be transmittable.  To assess the potential for trait transmission via wide crosses, a large number of maize-Tripsacum hybrids with differing chromosome numbers were screened in glasshouse experiments in western Kenya.   Those plants with 15 or more Tripsacum chromosomes performed very well under conditions of Striga infestation. A range of maize-Tripsacum addition lines are being screened in the laboratory in order to determine whether the trait can be localised to a chromosome or group of chromosomes of Tripsacum.  This data will help ascertain how complex the trait is and help determine whether transmission of the trait from Tripsacum to maize is possible via wide crossing.  In the absence of wide crossing, introduction of the trait into maize may be possible but first we must identify those characters responsible for the interesting phenotype.  Thus, we are using a combined approach incorporating both proteomics and genomics in order to elucidate the basis of resistance.  


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