Biotechnology, Breeding and Seed Systems for African Crops
Biotechnology, Breeding and Seed Systems for African Crops: Research and Product Development that Reaches Farmers. A conference organized by the Rockefeller Foundation and Co-hosted by the Ugandan National Research Organization (NARO) at the Royal Botanical Beach Hotel, Entebbe, Uganda from 4 to 7 November 2002.
Full Conference Programme
Sunday, 3 November 2002
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Monday,
4 November 2002 8:00-9:00
Registration at Royal Botanical Beach Hotel 9:00-9:15
Welcome and Opening Remarks from NARO, Joseph
Mukiibi, Director General, NARO 9:15-9:30
Welcome and Opening Remarks from The Rockefeller Foundation. Gary
Toenniessen, Director of Food Security, The Rockefeller Foundation 9:30-9:50
Address from the Guest of Honor. Hon.
Wilberforce K. Mugerwa, Minister of Agriculture 9:50-10:30
Keynote Address. Gordon
Conway, President of The Rockefeller Foundation
10:30-10:50
Tea Break 10:50-11:15
Development of an electro-transformation protocol for cowpea.
I. Sithole-Niang,
R. Mundembe and R. Allison 11:15-11:40
Novel determinants of osmotic stress tolerance using the
monocotyledonous ressurection plant, Xerophyta viscose. S.G.
Mundree and J.A. Thomson 11:40-12:05
Structural analysis of transgene rearrangements and effects on
expression in transgenic maize plants generated by particle bombardment.
L. Mehlo 12:05-12:30
Utilization of molecular markers to characterise and map useful
genes of the cultivated African rice, Oryza
glaberrima. N.
Njiondjop, A. Alonge, S. McCouch and M.P. Jones 12:30-12:55
Engineering insect resistance into maize using Bacillus
thuringiensis (Bt) cryiac gene fused to the c-terminal (galactose-binding)
of the ricin B-chain. D. Gahakwa, L. Mehlo, A. Gatehouse, J.P. Du, N.T. Loc, J. Gatehous and
P. Christou 12:55-13:00
Summary of presentations by
Session Chairperson
13:00-14:00
Lunch 14:00-17:00
Begin Concurrent Sessions (I) in Biotechnology, Breeding and Seed
Systems Biotechnology
Concurrent Session I. Chairperson:
M. Gale 14:00-14:20
Genetic mapping of a dominant gene conferring resistance to the
Cassava Mosaic Disease. M. Fregene, A.O. Akano, A.G. Dixon, C. Mba and E. Barrera 14:20-14:40
Marker assisted selection deployment of the novel cassava mosaic
disease resistance gene (CMD2) in African cassava gene pools.
O. Ariyo, A.G. Dixon, N. Morante, H. Ceballos and M. Fregene 14:40-15:00
Resistance to African cassava mosaic virus conferred by a mutant
of the putative NTP-binding domain of the rep gene (AC1) in Nicotiana
benthamiana. A.
Sangaré 15:00-15:20
RAPD, SSR and AFLP markers linked to genes conferring resistance
to angular leaf spot in common bean. G. Mahuku, C. Montoya, Y. Mantilla, M. Contreras, C. Jara and S. Beebe
15:20-15:40
Tea break
15:40-16:00
Poster Session 16:00-16:20
Distribution of angular leaf spot of common bean in Kenya and
virulence diversity of the causal agent, Phaeoisariopsis griseola. I.N.
Wagara, A.W. Mwangómbe, J.W. Kimenju and R.A. Buruchara
16:20-16:40
Adaptation of specific pcr based markers to characterize and
differentiate Pythium spp
associated with pythium bean root rot.
R. Buruchera, G. Mahuku, S.
Mayanja, J. Mukalazi and A. Levesque 16:40-17:00
Towards a maize based edible vaccine: LT-B producing maize
protects mice against Escherichia
coli heat labile enterotoxin (LT) and cholera toxin (CT).
R. Chikwamba, J. Cunnick,
D. Hathaway, J. McMurray, J. Mason and K. Wang Breeding
Concurrent Session I.
Chairperson: G. Ejeta
14:00-14:20
Breeding weevil-resistant maize: Is it feasible?
K. Pixley, T. Dhliwayo and S. Munjoma S. 14:20-14:40
Regionalized technology development and dissemination: The case
of breeding for drought tolerant maize varieties in Tanzania. Z.O.
Mduruma 14:40-15:00
Ecologically adapted maize open polinated varieties development
and screening in Zimbabwe. X. Mhike and L. Muza 15:00-15:20
Research of multiple disease resistant maize varieties in Malawi.
P.T.
Khonje, P. Ngwira, K.V. Pixley, G.W. Nhlane and V.H. Kabambe
15:20-15:40
Tea break
15:40-16:00
Poster Session
16:00-16:20
Maize breeding for alleviating production constraints in
Mozambique. P.
Fato, P. Chauque, D. Mariote, M. Langa and M. Denic
16:20-16:40
Guinea sorghum hybrids: Bringing the benefits of hybrid
technology to a staple crop of Sub-Saharan Africa.
A. Toure, F. Rattunde, D.
Sansan and E. Weltzien 16:40-17:00
Introgression of genes for Striga resistance into African
landraces of sorghum. I.
Kapran, C. Grenier, A. Elliot, A. Touré, Z. Gutema, A. Babiker, H.
Sadaan and G. Ejeta. Seed
Systems Concurrent Session I.
Chairperson: R. Tripp
14:00-14:20
Experiences with on-farm testing in maize variety evaluation in
Uganda. G. Bigirwa, G. Kikafunda,
J. Imanywoha, P. Kibwika, S. Mugo, M. Siambi, D. Beck and A.O. Diallo
14:20-14:40
Towards sustainable seed production and supply systems: Lessons
from the literature and a maize seed project in western Kenya and
Uganda. D. Beck, S. Mugo, M. Siambi and G. Bigirwa 14:40-15:00
Maize variety testing through the mother and baby design.
M.S. Mwala, J. de Meyer and M. Banziger 15:00-15:20
Transferring research results and technologies to end-users in
Kenya and Tanzania: A case study from the tissue culture banana project.
S.W.
Wakhusama, M.G. Karembu, F.N. Nguthi, A.S. Mbwana, M.M. Njuguna,
L.W. Njuguna and S.B. Patel 15:20-15:40
Tea break 15:40-16:00
Poster Session 16:00-16:20
Poverty reduction through introduction of tissue cultured bananas
in central Kenya. E.
Kahangi 16:20-16:40
Amélioration variétale pour la riziculture de bas-fond à
partir de variétés locales de riz Oryza glaberrima. F.
Cisse and Y. Doumbia 16:40-17:00
The potential of upland rice varieties in sustainable commercial
production of rice among small-holder farmers in Uganda.
P. Kibwika, J. Imanywoha,
G. Bigirwa and J. Kikafunda
End of Concurrent Sessions I 18:00-19:30
Reception and Entertainment 19:30
Dinner Tuesday,
5 November 2002 8:30-9:00
Progress in delivering stress-tolerant maize varieties in
southern Africa. M.
Bänziger, J. de Meyer, M. Mwala,
A. Phiri, B. Vivek and K. Pixley
9:00-9:30
Development of stress-tolerant maize varieties in Uganda.
J. Imanywoha, D.T. Kyetere, G. Bigirwa, J.T. Kikafunda, T.M. Kalule, K.
Pixley and A. Nakayima 9:30-10:00
Coordinated ecosystem breeding in Kenya:
Progress, challenges and opportunities.
J. Ininda, M. Gethi,
O. Odongo, J. Njuguna and J.A.W. Ochieng’ 10:00-10:30
Characterization and use of diversity within guinea-race
sorghums.
F.
Rattunde, A. Toure, E. Weltzien and D. Sansan 10:30-10:50
Tea break 10:50-11:20
Participatory plant breeding in Africa:
A framework for understanding diverse approaches and results
achieved. L.
Sperling 11:20-11:50
Towards improved East African highland banana genotypes with
resistance to pests and diseases. W.K. Tushereirwe, M. Pillay, M. Batte, T. Ssali, D. Talengera, K.
Nowakunda, J. Namaganda and A. Kiggundu 11:50-12:20
Status of participatory varietal selection for rice in Nigeria
and Mali. R.
Guei, H. Gridley, M. Jones and M. Worperies-Mura 12:20-12:50
The African center for crop improvement:
A new model for developing Ph.D. graduates in Africa.
D. M. Laing 12:50-13:00
Summary of presentations by
Session Chairperson 13:00-14:00
Lunch 14:00-17:00
Begin Concurrent Sessions (II) in Biotechnology, Breeding and
Seed Systems Biotechnology
Concurrent Session II.
Chairperson: P. Quail 14:00-14:20
Marker-assisted selection for maize improvement - practical
examples and strategies. J.M. Ribaut, M.
Banziger, K. Pixley and D. Hoisington 14:20-14:40
Striga free maize: Introducing tripsacum-based resistance via an
integrated approach. S.J.
Hearne, A.L. Gurney, J.P. Brizard, C. Brugidou, F. Kanampiu, P.O. Mbogo,
M.C. Press, J.D. Scholes
and D. Hoisington 14:40-15:00
Generating Striga resistant maize: Identification of sources of
resistance in wild relatives. A.L
Gurney, D. Grimanelli, S.J. Hearne, F. Kanampiu, D. Hoisington, J.D.
Scholes and M.C. Press 15:00-15:20
Can wild relatives of maize provide new sources of resistance to
the root hemi-parasite Striga hermonthica? J.D.
Scholes, A.L. Gurney, D. Grimanelli, D. Hoisington and M.C. Press
15:20-15:40
Tea break
15:40-16:00
Poster Session 16:00-16:20
Genetic analysis of resistance to Striga
hermonthica introgressed from Zea diploperennis to tropical maize.
O. Menkir, A. Melake-Berhan, J.G.
Kling and O. Ibikunle 16:20-16:40
A search for random amplified polymorphic DNA (RAPD) and simple
sequence repeat (SSR) markers that are linked to Striga
asiatica resistance in sorghum.
C.S. Mutengwa, P. Tongoona, Z. Dhlamini and I. Sithole-Niang
16:40-17:00
Bioassays to characterize and dissect mechanisms of resistance to
Striga. C.
Grenier, A. Mohammed, P.J. Rich, T. Housley and E. Ejeta
Breeding
Concurrent Session II.
Chair:
W.R. Coffman 14:00-14:20
Genetic variation, utilization and potential of African landraces
in the genetic improvement of cassava in Sub-Saharan Africa.
A.G.O. Dixon, J.B.A.
Whyte, N.M. Mahuku, B.W. Khizzah, M. Fregene, A.A. Akano and M.
Gedil 14:20-14:40
Regional germplasm development and deployment for sustainable
production and utilization within the cassava sub-sector.
J.B.A. Whyte, B.W. Khizzah, A.G.O. Dixon, N.M. Mahungu, P. Ragama, P. Ntawuruhunga and
H. Ojulong 14:40-15:00
Evaluation of cassava clones for yield, acceptability and disease
and pest resistance in coastal Kenya.
T.L. Munga
15:00-15:20
Establishment of an insectary and advances in rearing of maize
stemborers at KARI, Katumani. J.M. Songa and D.
Mutisya 15:20-15:40
Tea break
15:40-16:00
Poster Session 16:00-16:20
Heterosis in maize hybrids: Fact or artefact?
J.A.W. Ochieng 16:20-16:40
Interactive maize breeding for biotic stresses across ecosystems
in Kenya. O.M.
Odongo, S.I. Ajanga, C.M. Mburu and J. Ininda
16:40-17:00
Introgression of resistance to Striga hermonthica and Striga
asiatica from wild relatives and selections in maize.
J. Gethi Seed
Systems Concurrent Session II. Chairperson:
G. Ejeta 14:00-14:20
Involving farmers in varietal selection: Experiences from the
seed project work in western Kenya. M. Siambi, G. Odhiambo,
S. Mugo, O. Odongo, A. Diallo, D. Beck and H. DeGroote 14:20-14:40
Country wide maize variety evaluation using the mother-baby
scheme in Zimbabwe. L.
Muza, D. Ruponga, V. Kazembe, E. Nyamutowa
and J. de Meyer 14:40-15:00
Working with a women’s farmer association in maize seed
production.
J. Kikafunda, G. Bigirwa, J. Imanywoha, S. Mugo and D. Beck
15:00-15:20
Evaluation and dissemination of introduced banana germplasm:
preliminary assessment of the planting material distribution system.
A.K. Nowakunda 15:20-15:40
Tea break
15:40-16:00
Poster Session 16:00-16:20
Use of simple technology (good seed and cheap water) to boost
food security and alleviate poverty.
A.M. Mwololo, T. Kambura
and D. Nzyuko 16:20-16:40
Evaluation of
selected beta-carotene rich sweetpotato varieties for improving vitamin
a nutrition in Iganga and Kamuli districts of Uganda.
J.M. Ssebuliba, J.H.
Muyonga and W. Ekere 16:40-17:00
Involving farmers in maize variety selection and seed production:
experiences from the lake region of western Kenya.
G.D. Odhiambo, M. Siambi, R. Apamo, E. Ochieng, P. Mbai, S. Mugo, and H.
DeGroote.
End of Concurrent Sessions II 19:00-20:00
Dinner 20:30-22:00
Evening session on biotechnology and biosafety policy development
in sub-Saharan Africa. W. Goredema, P.
Kameri-Mbote, L. Kitch and J. Webster Wednesday,
6 November 2002 08:00-17:00
Field trip to NARO research stations at Kawanda and Namulonge 19:00-21:00
Reception hosted by NARO at The Windsor Lake Victoria Hotel,
Entebbe Thursday,
7 November 2002 8:30-9:00
Developing the smallholder seed market in Uganda:
The Harvest Farms Seed Company Experience.
J.A. Okot 9:00-9:30
Opportunities and issues in private seed supply in Africa.
F. Mohammed 9:30-10:00
Development of adapted and high-yielding maize varieties for the
stressful Lake Victoria Region. M.J.F.
Onim and M. Dida 10:00-10:30
Towards sustainable maize seed production and supply systems in
western Kenya and Uganda. S. Mugo, M. Siambi, G.
Bigirwa, G. Odhiambo, D. Beck, A. Diallo and P. Kibwika 10:30-10:50
Tea break 10:50-11:20
Striga weed control in maize using herbicide seed coating
technology. F.K. Kanampiu, J.K. Ransom, D.K.
Friesen and J. Gressel 11:20-11:50
Germplasm exchange and seed multiplication of cassava in Kenya:
Challenges and opportunities. H.M. Obiero, P.J. Ndolo, M.S. Akhwale and K.. Orondo 11:50-12:20
Assessment of marketing and utilization of improved maize seed in
southern Africa: The case
of Malawi, Tanzania, Zambia, and Zimbabwe.
M.A.R.
Phiri 12:20-12:50
Alternative biotechnologies for controlling Striga: the next
generations. J.
Gressel 12:50-13:00
Presentation Summary by Session Chair 13:00-14:00
Lunch 14:00-17:00
Begin Concurrent Sessions (III) in Biotechnology and Breeding Biotechnology
Concurrent Session IIIa.
Chairperson: J. Kling 14:00-14:20
Multiple Cry genes for avoiding build-up of resistance among
insects to Bt toxins: Learning from Bt-rice.
I. Altosaar and M. Zaidi 14:20-14:40
Will transformation systems for the large seeded grain legumes,
peas, lupins or chickpeas be adaptable to cowpea? T.J.V.
Higgins, L. Molvig, W. Tate, S. Gollasch and L.M. Tabe
14:40-15:00
Evaluation of cowpea aphid-borne mosaic virus coat protein and
RNA mediated resistance strategies in Nicotiana
benthamiana. R. Mundembe and
I. Sithole-Niang 15:00-15:20
Gene flow between cultivated and wild cowpea in Kenya.
R.S. Pasquet, A. Ochieng’ and K. Thoen
15:20-15:40
Tea break 15:40-16:00
The genetics of virulence of the maize streak mastrevirus. R.
Edema, D.T. Gordon and D.M. Bisaro
16:00-16:20
Preliminary results on surveys of banana streak badnavirus (BSV)
in Kenya. A.
Wangai and L. Karanja 16:20-16:40
Development of highland banana cell suspension system; a critical
stage in genetic improvement of the banana.
P. Namanya, G.Mutumba, S.M.
Magambo and W. Tushemereirwe Biotechnology
Concurrent Session IIIb.
Chairman: P. Quail 14:00-14:20
Development of Agrobacterium-ediated clean gene (marker free)
technology for rice transformation using a novel dual binary plasmid
pgreen/psoup. A.S.
Afolabi, J. Snape and P. Vain 14:20-14:40
Molecular characterization of a novel stress-inducible
antioxidant gene, xvper1, from the resurrection plant Xerophyta
viscose Baker.
S. B. Mowla, J. A. Thomson, J. M. Farrant and S. G. Mundree.
14:40-15:00
Review of tissue culture and genetic studies of East African
Highland bananas in Uganda. P.R. Rubaihayo, S.
Kassim, S. Nanteza and A.K. Tugume 15:00-15:20
Control of the witchweed Striga
hermonthica by intercropping with Desmodium
spp. Z.R. Khan, A. Hassanali, W. Overholt, T.M. Khamis, J.A. Pickett and L.J.
Wadhams 15:20-15:40
Tea Break 15:40-16:00
Chemistry of Desmodium root exudates and Striga
control: research status and prospects.
A. Hassanali, T.K. Muniru,
A.M. Hooper, Z.R. Khan, F. Kaberia, J.A.
Pickett and L.J. Wadhams 16:00-16:20
Allelochemical mechanisms of Striga
hermonthica suppression by Desmodium
uncinatum. J. A.
Pickett, Z.R. Khan, A. Hassanali, W. Overholt, T.M. Khamis, A.M. Hooper,
L.J. Wadhams and C.M. Woodcock 16:20-16:40
Biotechnology applications in breeding for Striga
asiatica resistance at the University of Zimbabwe.
P.B. Tongoona, C.S.
Mutengwa, C. Musimwa, I. Sithole-Niang and A. Matibiri Breeding
Concurrent Session III.
Chairperson: M. Jones 14:00-14:20
The impact of root rot resistant bush and climbing bean varieties
on household livelihoods in Western Kenya.
S. David, M. Odendo and R.
Otysula 14:20-14:40
Breeding bean cultivars resistant to angular leafspot and root
rots in Eastern and Central Africa.
P.M. Kimani, R. Buruchara,
R. Otsyula and G. Rachie 14:40-15:00
Inheritance of angular leaf spot resistance and marker assisted
selection for disease resistance in common bean.
A. Namayanja, R. Buruchara,
P. Kimani, G. Mahuku and P. Rubaihayo 15:00-15:20
Breeding climbing beans resistant to angular leaf spot, pythium
root rots and fusarium wilt. A. Musoni, R. Buruchara
and P. Kimani 15:20-15:40
Tea break 15:40-16:00
Banana weevil resistance in musa and prospects for genetic
improvement. A.
Kiggundu, C.S. Gold, M.T. Labuschagne, D. Vuylsteke, S. Louw S, A.
Hassanali and W. Tushemereirwe 16:00-16:20
Regional disease nursery (REGNUR): A unique opportunity for
developing multiple-disease-resistant maize.
B. Vivek, K. Pixley, O.
Odongo, J. Njuguna, J. Imanywoha, G. Bigirwa and A. Diallo 16:20-16:40
Sources of resistance to seed –transmission and variation in
responses of cowpea varieties to infection by four seed- borne viruses.
S. A. Shoyinka, M.A. Ittah, I. Fawole and J.A. Hughes
End
of Concurrent Sessions III 16:50-17:30 Closing Session with statements by Hon. Isaac Musumba, Minister of State for Planning and Monty Jones, Chairman of the Scientific Advisory Committee
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