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Marker-assisted selection for maize streak virus resistance in elite maize lines G. Abalo, P. Tongoona, J. Imanywoha, K. Pixley, R. Edema Previous studies have identified a major quantitative trait locus (QTL) responsible for maize streak virus (MSV) resistance in the short arm of chromosome 1. This QTL, known as msv-1, accounts for between 48 and 62% of the total phenotypic variation of resistance, which makes it an attractive tool for marker-assisted selection (MAS). In an effort to develop an efficient MAS protocol for MSV resistance, 3 parent lines and their F1s were screened using 32 pairs of simple sequence repeat markers under a touchdown polymerase chain reaction (PCR) protocol. Two flanking markers, umc 1917 and umc 2228, were found consistently poly-morphic. The two were also within the acceptable genetic distance from each other. The effectiveness of these markers is now being tested in two segregating populations developed from crosses between CML 321 x CML 202 and CML 384 x CML 202. The results and discussions are presented. |