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PI:
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Idah
Sithole-Niang
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Contact details
Department
of Biochemistry
P.O. MP 167, Mt. Pleasant, Harare,
Zimbabwe
Phone:
+263 (-4) -303211 , Ext 1663
Fax:
+263 (-4) -333407 /333678
Email
direct from site:
isn@mweb.co.zw
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Grantee:
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University
of Zimbabwe
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Amount:
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US
$87,150
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Duration:
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Three
Years
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Collaborator:
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Dr.
Richard F Allison, Plant Biology, Michigan State University
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Project
Outputs
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Generated
a workable and novel genetic transformation system of cowpea based
on:
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enzymatic
expression of the marker gene GUS by ELISA detection,
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PCR
of the transgene (coat protein gene), and
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a
positive hybridization event on Southern blots. However no
sequencing has been done to verify the insertion sites, and no
virus challenge has been conducted to confirm resistance as yet.
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Four
transgenic cowpeas (Zimbabwean cultivar 475/89) identified by the
above criteria and awaiting evaluation for virus resistance
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Capacity
building:
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infrastructure
development: computer and gel documentation system, flourimeter,
incubator shaker and the growth chambers acquired
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human
resource development: further training for the student and
collaboration with Michigan State University
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Successful
negotiation of IPR waiver on patented technology, the first ever
negotiated in sub-Saharan Africa, and no money spent on the process
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Scientific
publications envisaged
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