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PI:
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Annette Namayanja
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Grantee:
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NARO
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Amount:
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US $41,000
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Duration:
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Three
Years
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Contact details
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NARO-Namulonge, P.O. Box 7084
, Kampala, Uganda;
Phone:
+256 (77) 430072
Fax:
+256 (41) 21070
Email
direct from site:
naari@afsat.com
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Project Outputs
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Inheritance studies have shown
that resistance genes in each of the cultivars Mexico 54 and BAT 332 to
pathotype 63 – 39, one of the most virulent forms of P.griseola
in Africa is due to a single dominant gene. Mexico 54 is resistant to
158 isolates of P. griseola out of the 163 so far characterized
in Africa. BAT is also a very good source of resistance.
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Studies to validate the utility
of markers indicated that the SCAR OPNO2890C was not
polymorphic for the Phg-2 gene outside the mapping population and
therefore not useful in selecting resistant genotypes in the study
populations. On the other hand, the RAPD marker was observed in all
resistant individuals and was absent in those scored susceptible based
on virulence (plate 1). Use of the RAPD marker OPE-04 has been initiated
and efforts are going on to convert it into a SCAR.
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Segregating populations for
resistance to angular leaf spot disease have been generated using
Mexico 54 and BAT 332 as resistant parents. The susceptible cultivars
included CAL 96 (K 132), Kanyebwa (popular land race), MCM 5001 (K 131),
K20 (GLP 2) and Urugezi. The segregating populations will be evaluated
with other partners in the region. In addition to these we have created
more segregating populations for multiple parent crosses, to
specifically combine angular leaf spot, common bacterial blight, root
rots and low soil fertility resistances.
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A backcrossing programme to
specifically incorporate angular leaf spot resistance to the commercial,
but popular susceptible cultivars (K 132, Kanyebwa, Urugezi and GLP 2
(K20) was initiated. K 132 is a released variety in Uganda, while
Kanyebwa is a popular land race. GLP 2 is grown in Kenya and in Uganda
it is known as K20. Urugezi is popular in Rwanda.
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Farmer participatory variety
selection activities were initiated in Kabale (S.W Uganda) and Namulonge.
The materials evaluated by farmers consisted of both advanced lines and
segregating populations. In Kabale, focus was on the root rot disease.
Farmers have selected some genotypes for subsequent evaluations on their
fields. Among the popular genotypes selected by farmers were RWR 2075,
RWR 1946 and RWR 1873 (these three genotypes have been observed to be
tolerant to bean root rot and originated from the BILFA III nursery).
Other popular selected genotypes were BACO 2 –4/6, BOA 1 –4/9 and BOA 5
–1/30.
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Partners
NARO
CIAT
Makerere University
Farmers
District extension – Kabale and Wakiso
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